General Information

Cytidine Impurities and Cytidine

Daicel Pharma synthesizes Cytidine impurities of exceptional quality, such as Cytidine 2-Ethylbutyl Impurity (R-Isomer), Cytidine 2-Ethylbutyl Impurity (S-Isomer), Cytidine 2-Ethylbutyl Sesamol Impurity (R-Isomer), Cytidine 2-Ethylbutyl sesamol Impurity (S-Isomer), Cytidine Isopropyl Impurity, Cytidine Isopropyl Impurity (R-Isomer), Cytidine Isopropyl Impurity (S-Isomer) and L-Cytidine. These impurities are crucial to assess the purity, reliability, and safety of Cytidine, an active pharmaceutical ingredient. Besides, Daicel Pharma provides custom synthesis of Cytidine impurities to meet clients’ demands for delivery worldwide.

Cytidine [CAS: 65-46-3] is a pyrimidine nucleoside. It comprises attaching cytosine to ribofuranose through a beta-N(1)-glycosidic bond. It is an RNA component and plays a role in various physiological processes.

Cytidine: Use and Commercial Availability  

Cytidine treats neuropsychiatric impairments associated with cerebrovascular diseases, often in combination with uridine, to enhance therapeutic effects. It serves as a pyrimidine compound that can be incorporated into nucleic acids and also acts as a substrate for the salvage pathway of pyrimidine nucleotide synthesis. The enzyme CTP synthase 1 (CTPS1) helps form Cytidine triphosphate, a vital precursor for DNA, RNA, and phospholipids.

Cytidine Structure and Mechanism of Action

The chemical name of Cytidine is 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-4-(amino)pyrimidin-2(1H)-one. Its chemical formula is C₉H₁₃N₃O₅, and its molecular weight is approximately 243.22 g/mol.

The mechanism of action of Cytidine is unknown.

Cytidine Impurities and Synthesis

Impurities in Cytidine can arise from various sources, including manufacturing¹, raw materials, or degradation during storage. They can impact the purity and quality of Cytidine, potentially affecting its performance and safety. So, it is necessary to analyze and control these impurities. The common Cytidine impurities may include related substances, degradation products, and residual solvents. Analytical methods such as HPLC or LC help detect and quantify these impurities, ensuring that Cytidine meets the required quality standards for its intended use in research or pharmaceutical applications.

Daicel Pharma offers a Certificate of Analysis (CoA) for Cytidine impurity standards, such as Cytidine 2-Ethylbutyl Impurity (R-Isomer), Cytidine 2-Ethylbutyl Impurity (S-Isomer), Cytidine 2-Ethylbutyl Sesamol Impurity (R-Isomer), Cytidine 2-Ethylbutyl sesamol Impurity (S-Isomer), Cytidine Isopropyl Impurity, Cytidine Isopropyl Impurity (R-Isomer), Cytidine Isopropyl Impurity (S-Isomer) and L-Cytidine, generated from an analytical facility compliant with cGMP standards. The CoA includes a comprehensive characterization report comprising data from techniques like 1H NMR, 13C NMR, IR, MASS, and HPLC purity. Furthermore, on request, we give additional data like 13C-DEPT and CHN. Daicel Pharma can synthesize unknown Cytidine impurities or degradation products. A complete characterization report accompanies every delivery.